Characteristic of K3 (CpG-ODN) as a Transcutaneous Vaccine Formulation Adjuvant

Characteristic of K3 (CpG-ODN) as a Transcutaneous Vaccine Formulation Adjuvant

Blog Article

Transcutaneous immunization (TCI) is easy to use, minimally invasive, and has excellent efficacy in vaccines against infections.We focused on animed blue lotion topical spray toll-like receptor (TLR) ligands as applicable adjuvants for transcutaneous formulations and characterized immune responses.TCI was performed using poke-and-patch methods, in which puncture holes are formed with a polyglycolic acid microneedle on the back skin of mice.

Various TLR ligands were applied to the puncture holes and covered with an ovalbumin-loaded hydrophilic gel patch.During the screening process, K3 (CpG-oligonucleotide) successfully produced more antigen-specific antibodies than other TLR ligands and induced T helper (Th) 1-type polarization.Transcutaneously administered K3 was detected in draining lymph nodes and was found to promote B cell activation and differentiation, suggesting a direct transcutaneous adjuvant activity on B cells.

Furthermore, a human safety test of K3-loaded self-dissolving microneedles (sdMN) was performed.Although a local skin reaction was observed at the sdMN application site, there was no systemic side reaction.In summary, we report a K3-induced Th1-type immune response that is a promising adjuvant for transcutaneous vaccine formulations using MN and show that K3-loaded sdMN shiro neon zero can be safely applied to human skin.